Plasmid_Backbone

Part:BBa_K516999

Designed by: Nicolo' Politi, Susanna Zucca, Tommaso Goggia, Paolo Magni   Group: iGEM11_UNIPV-Pavia   (2011-09-15)

pSB1C3 with RBS-RFP-TT between SpeI-PstI

When the SpeI-PstI DNA fragment from BBa_J61002, containing the mRFP1 gene with a strong RBS and a double terminator, is transferred into pSB1C3 (digested SpeI-PstI) the resulting plasmid is BBa_K516999. See pSB1C3 for detailed feature information.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2918
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal NotI site found at 878
    Illegal NotI site found at 2924
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2918
    Illegal XhoI site found at 1902
    Illegal XhoI site found at 2794
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2918
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2918
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2933
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
n/apSB1C3 with RBS-RFP-TT between SpeI-PstI